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suman vardhana
Query: 1 / Answer: 3

25th Aug 2019 .

Write the steps you would suggest to be undertaken to obtain a foreign-gene-product. 

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Komal Abasti
Query: 2 / Answer: 2

25th Aug 2019 .

Recombinant DNA technology can be used to obtain foreign-gene-products. It involves following steps :
(i) Isolation of genetic material (DNA) using enzymes.
(ii) Identification and cutting of DNA from specific location using restriction enzymes and separating the fragments of DNA using gel electrophoresis to obtain gene of interest.
(iii) Amplification of gene of interest using PCR.
(iv) Adding or ligation of gene of interest into suitable vector using ligase enzymes. This produces a recombinant DNA molecule.
(v) Insertion of recombinant DNA into the host cell or organism.
(vi) Selection of recombinants from non- recombinants cells.
(vi) Culturing recombinant cell under suitable conditions and obtaining the desired foreign-gene- product
 
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