(a) Positive terminal (anode) — B
Negative terminal (cathode) —A
(b) DNA is negatively charged molecule. In electrophoresis, molecules are separated unless the influence of electrical field, i.e., negatively charged DNA moves towards positively charged anode through a medium.
(c) The separated DNA fragments can be seen only after staining the DNA with a compound known as ethidium bromide (EtBr) followed by exposure to UV radiation as bright orange coloured bands. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This process called as elution (removal of adsorbent).
