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Kumari Akansha sharma
Query: 2 / Answer: 1

07th Sep 2019 .

Explain the various steps involved in the production of artificial insulin.

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Ankita Baiju
Query: 2 / Answer: 2

07th Sep 2019 .

The steps involved in the production of artificial insulin or humulin are as follows:
 
(i) Isolation of donor or DNA segment – A useful DNA segment is isolated from the donor organism.
(ii) Formation of recombinant DNA (rDNA) – Both the vector and donor DNA segments are cut in the presence of restriction endonuclease. In the presence of ligase DNA segments of both are joined to form rDNA.
(iii) Production of multiple copies of rDNA – In this process multiple copies of this recombinant DNA are produced.
(iv) Introduction of rDNA in the recipient organism
– The rDNA is inserted into a recipient organism.
(v) Screening of the transformed cells – The recipient (host) cells are screened in the presence of rDNA and the product of donor gene. The transformed cells are separated and multiplied.
 
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